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mouse anti synapsin iia  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mouse anti synapsin iia
    Mouse Anti Synapsin Iia, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 10 article reviews
    mouse anti synapsin iia - by Bioz Stars, 2026-03
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    a The represent image of fluorescence microscope. The polymer statu of JC-1 indicate the higher MMP (red) and the monomer statu of JC-1 indicate the lower MMP (green). b Ratio of red fluorescence to green fluorescence. c ATP levels from neuronal lysates. d Confocal microscopy of cultured neurons using a PDH antibody (green) and Map-2 antibody (red). The boxed area in each subpanel is magnified below its corresponding subpanel and shows neurites. e Mean mitochondrial aspect ratio in the box areas in c . f Quantitative analysis of the green fluorescent intensity in the boxed area (1367.02 μm length of neurites from the control group, 1058.74 μm length of neurites from the Fen group). g In vivo and h in vitro protein levels of PSD-95, synaptotagmin, and <t>synapsin</t> detected by Western blotting (top panel) and quantitative analysis of protein-band intensities are shown (bottom panel). i Averaged mEPSCs from an example recording are shown (left panel), and quantitative analysis of frequency and amplitude are shown (right panel). Figure d and h are represent same samples, therefore, Actin in Fig. d and h are completely identical. For all quantitative/statistical analysis, * p < 0.05; ** p < 0.01; and *** p < 0.001. All data are presented as means ± SEM. Scale bars are 20 μm in c .
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    a The represent image of fluorescence microscope. The polymer statu of JC-1 indicate the higher MMP (red) and the monomer statu of JC-1 indicate the lower MMP (green). b Ratio of red fluorescence to green fluorescence. c ATP levels from neuronal lysates. d Confocal microscopy of cultured neurons using a PDH antibody (green) and Map-2 antibody (red). The boxed area in each subpanel is magnified below its corresponding subpanel and shows neurites. e Mean mitochondrial aspect ratio in the box areas in c . f Quantitative analysis of the green fluorescent intensity in the boxed area (1367.02 μm length of neurites from the control group, 1058.74 μm length of neurites from the Fen group). g In vivo and h in vitro protein levels of PSD-95, synaptotagmin, and <t>synapsin</t> detected by Western blotting (top panel) and quantitative analysis of protein-band intensities are shown (bottom panel). i Averaged mEPSCs from an example recording are shown (left panel), and quantitative analysis of frequency and amplitude are shown (right panel). Figure d and h are represent same samples, therefore, Actin in Fig. d and h are completely identical. For all quantitative/statistical analysis, * p < 0.05; ** p < 0.01; and *** p < 0.001. All data are presented as means ± SEM. Scale bars are 20 μm in c .
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    a The represent image of fluorescence microscope. The polymer statu of JC-1 indicate the higher MMP (red) and the monomer statu of JC-1 indicate the lower MMP (green). b Ratio of red fluorescence to green fluorescence. c ATP levels from neuronal lysates. d Confocal microscopy of cultured neurons using a PDH antibody (green) and Map-2 antibody (red). The boxed area in each subpanel is magnified below its corresponding subpanel and shows neurites. e Mean mitochondrial aspect ratio in the box areas in c . f Quantitative analysis of the green fluorescent intensity in the boxed area (1367.02 μm length of neurites from the control group, 1058.74 μm length of neurites from the Fen group). g In vivo and h in vitro protein levels of PSD-95, synaptotagmin, and <t>synapsin</t> detected by Western blotting (top panel) and quantitative analysis of protein-band intensities are shown (bottom panel). i Averaged mEPSCs from an example recording are shown (left panel), and quantitative analysis of frequency and amplitude are shown (right panel). Figure d and h are represent same samples, therefore, Actin in Fig. d and h are completely identical. For all quantitative/statistical analysis, * p < 0.05; ** p < 0.01; and *** p < 0.001. All data are presented as means ± SEM. Scale bars are 20 μm in c .
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    a The represent image of fluorescence microscope. The polymer statu of JC-1 indicate the higher MMP (red) and the monomer statu of JC-1 indicate the lower MMP (green). b Ratio of red fluorescence to green fluorescence. c ATP levels from neuronal lysates. d Confocal microscopy of cultured neurons using a PDH antibody (green) and Map-2 antibody (red). The boxed area in each subpanel is magnified below its corresponding subpanel and shows neurites. e Mean mitochondrial aspect ratio in the box areas in c . f Quantitative analysis of the green fluorescent intensity in the boxed area (1367.02 μm length of neurites from the control group, 1058.74 μm length of neurites from the Fen group). g In vivo and h in vitro protein levels of PSD-95, synaptotagmin, and <t>synapsin</t> detected by Western blotting (top panel) and quantitative analysis of protein-band intensities are shown (bottom panel). i Averaged mEPSCs from an example recording are shown (left panel), and quantitative analysis of frequency and amplitude are shown (right panel). Figure d and h are represent same samples, therefore, Actin in Fig. d and h are completely identical. For all quantitative/statistical analysis, * p < 0.05; ** p < 0.01; and *** p < 0.001. All data are presented as means ± SEM. Scale bars are 20 μm in c .
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    a The represent image of fluorescence microscope. The polymer statu of JC-1 indicate the higher MMP (red) and the monomer statu of JC-1 indicate the lower MMP (green). b Ratio of red fluorescence to green fluorescence. c ATP levels from neuronal lysates. d Confocal microscopy of cultured neurons using a PDH antibody (green) and Map-2 antibody (red). The boxed area in each subpanel is magnified below its corresponding subpanel and shows neurites. e Mean mitochondrial aspect ratio in the box areas in c . f Quantitative analysis of the green fluorescent intensity in the boxed area (1367.02 μm length of neurites from the control group, 1058.74 μm length of neurites from the Fen group). g In vivo and h in vitro protein levels of PSD-95, synaptotagmin, and <t>synapsin</t> detected by Western blotting (top panel) and quantitative analysis of protein-band intensities are shown (bottom panel). i Averaged mEPSCs from an example recording are shown (left panel), and quantitative analysis of frequency and amplitude are shown (right panel). Figure d and h are represent same samples, therefore, Actin in Fig. d and h are completely identical. For all quantitative/statistical analysis, * p < 0.05; ** p < 0.01; and *** p < 0.001. All data are presented as means ± SEM. Scale bars are 20 μm in c .
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    a The represent image of fluorescence microscope. The polymer statu of JC-1 indicate the higher MMP (red) and the monomer statu of JC-1 indicate the lower MMP (green). b Ratio of red fluorescence to green fluorescence. c ATP levels from neuronal lysates. d Confocal microscopy of cultured neurons using a PDH antibody (green) and Map-2 antibody (red). The boxed area in each subpanel is magnified below its corresponding subpanel and shows neurites. e Mean mitochondrial aspect ratio in the box areas in c . f Quantitative analysis of the green fluorescent intensity in the boxed area (1367.02 μm length of neurites from the control group, 1058.74 μm length of neurites from the Fen group). g In vivo and h in vitro protein levels of PSD-95, synaptotagmin, and <t>synapsin</t> detected by Western blotting (top panel) and quantitative analysis of protein-band intensities are shown (bottom panel). i Averaged mEPSCs from an example recording are shown (left panel), and quantitative analysis of frequency and amplitude are shown (right panel). Figure d and h are represent same samples, therefore, Actin in Fig. d and h are completely identical. For all quantitative/statistical analysis, * p < 0.05; ** p < 0.01; and *** p < 0.001. All data are presented as means ± SEM. Scale bars are 20 μm in c .
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    Image Search Results


    a The represent image of fluorescence microscope. The polymer statu of JC-1 indicate the higher MMP (red) and the monomer statu of JC-1 indicate the lower MMP (green). b Ratio of red fluorescence to green fluorescence. c ATP levels from neuronal lysates. d Confocal microscopy of cultured neurons using a PDH antibody (green) and Map-2 antibody (red). The boxed area in each subpanel is magnified below its corresponding subpanel and shows neurites. e Mean mitochondrial aspect ratio in the box areas in c . f Quantitative analysis of the green fluorescent intensity in the boxed area (1367.02 μm length of neurites from the control group, 1058.74 μm length of neurites from the Fen group). g In vivo and h in vitro protein levels of PSD-95, synaptotagmin, and synapsin detected by Western blotting (top panel) and quantitative analysis of protein-band intensities are shown (bottom panel). i Averaged mEPSCs from an example recording are shown (left panel), and quantitative analysis of frequency and amplitude are shown (right panel). Figure d and h are represent same samples, therefore, Actin in Fig. d and h are completely identical. For all quantitative/statistical analysis, * p < 0.05; ** p < 0.01; and *** p < 0.001. All data are presented as means ± SEM. Scale bars are 20 μm in c .

    Journal: Cell Death Discovery

    Article Title: Fenpropathrin induces degeneration of dopaminergic neurons via disruption of the mitochondrial quality control system

    doi: 10.1038/s41420-020-00313-y

    Figure Lengend Snippet: a The represent image of fluorescence microscope. The polymer statu of JC-1 indicate the higher MMP (red) and the monomer statu of JC-1 indicate the lower MMP (green). b Ratio of red fluorescence to green fluorescence. c ATP levels from neuronal lysates. d Confocal microscopy of cultured neurons using a PDH antibody (green) and Map-2 antibody (red). The boxed area in each subpanel is magnified below its corresponding subpanel and shows neurites. e Mean mitochondrial aspect ratio in the box areas in c . f Quantitative analysis of the green fluorescent intensity in the boxed area (1367.02 μm length of neurites from the control group, 1058.74 μm length of neurites from the Fen group). g In vivo and h in vitro protein levels of PSD-95, synaptotagmin, and synapsin detected by Western blotting (top panel) and quantitative analysis of protein-band intensities are shown (bottom panel). i Averaged mEPSCs from an example recording are shown (left panel), and quantitative analysis of frequency and amplitude are shown (right panel). Figure d and h are represent same samples, therefore, Actin in Fig. d and h are completely identical. For all quantitative/statistical analysis, * p < 0.05; ** p < 0.01; and *** p < 0.001. All data are presented as means ± SEM. Scale bars are 20 μm in c .

    Article Snippet: The following antibodies and chemicals were used in this study: TH mouse monoclonal antibody (Santa Cruz Biotechnology, sc-25269), HO-1 mouse antibody (ProteinTech, 10701-1-AP), SOD-1 mouse monoclonal antibody (Santa Cruz Biotechnology, sc-101523), Drp1 rabbit monoclonal antibody (EnoGene, E2A-7037), Mfn-1 and Mfn-2 rabbit polyclonal antibody (BOSTER Biotechnology, PB0263 and BA1790-2), PDH mouse monoclonal antibody (Santa Cruz Biotechnology, sc-377092), p62/SQSTM1 rabbit polyclonal antibody (Cell Signaling Technology, #23214), LC3A/B rabbit polyclonal antibody (Cell Signaling Technology, #12741), PINK1 rabbit polyclonal antibody (Affinity Biotechnology, DF7742), MitoTracker (ThermoFisher Scientific, M22425), LysoTracker (ThermoFisher Scientific, L7528), PSD-95 rabbit polyclonal antibody (Cell Signaling Technology, #3450), synaptotagmin rabbit polyclonal antibody (Cell Signaling Technology, #14558), synapsin mouse monoclonal antibody (Santa Cruz Biotechnology, sc-136086), 4-HNE Monoclonal Mouse IgG 2B antibody (R&D Systems, MAB3249), actin Monoclonal Mouse antibody (Beyotime Biotechnology, AA128), horseradish peroxidase-conjugated goat anti-mouse (Beyotime Biotechnology, A0216), horseradish peroxidase-conjugated goat anti-rabbit (Beyotime Biotechnology, A0208), anti-Alexa Fluor 488-conjugated goat anti-mouse (BOSTER Biotechnology, BA1126), anti-Alexa Fluor 555-conjugated goat anti-rabbit (Boster Biotechnology, BA1135), reactive oxygen species assay kit (Beyotime Biotechnology, S0033), mitochondrial membrane potential assay kit with JC-1 (Beyotime Biotechnology, C2006), enhanced ATP Assay Kit (Beyotime, S0027), total superoxide dismutase assay kit with WST-8 (Beyotime, S010), fenpropathrin (Dr. Ehrensorfer, C13530000), NAC (Beyotime, S0077), CCCP (Solarbio Life Sciences, C6700), CQ (Sigma-Aldrich, C6628), and Restore-Plus Western-Blot stripping Buffer (ThermoFisher Scientific, 46430).

    Techniques: Fluorescence, Microscopy, Polymer, Confocal Microscopy, Cell Culture, Control, In Vivo, In Vitro, Western Blot